فایل ورد (word) مقاله Curcumin Inhibits TGF-?1-Induced MMP-9 and Invasion through ERK and Smad Signaling in Breast Cancer MDAMB- 231 Cells

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بخشی از متن فایل ورد (word) مقاله Curcumin Inhibits TGF-?1-Induced MMP-9 and Invasion through ERK and Smad Signaling in Breast Cancer MDAMB- 231 Cells :


سال انتشار : 2012

تعداد صفحات :10

Objective: To evaluate the effects of curcumin on matrixmetalloproteinase-9 (MMP-9) and invasion abilityinduced by transforming growth factor-1 (TGF-1) in MDA-MB-231 cells and potential mechanisms. Methods:Human breast cancer MDA- MB-231 cells were used with the CCK-8 assay to measure the cytotoxicity ofcurcumin. After treatment with 10 ng/ml TGF-1, with or without curcumin (10 M), cell invasion was checkedby transwell chamber. The effects of curcumin on TGF-1-stimulated MMP-9 and phosphorylation of Smad2,extracellular-regulated kinase (ERK), and p38 mitogen activated protein kinases (p38MAPK) were examined byWestern blotting. Supernatant liquid were collected to analyze the activity of MMP-9 via zymography. Followingtreatment with PD98059, a specific inhibitor of ERK, and SB203580, a specific inhibitor of p38MAPK, Westernblotting and zymography were employed to examine MMP-9 expression and activity, respectively. Results: Lowdose curcumin (10 M) did not show any obvious toxicity to the cells, while 0~10 mol/L caused a concentration–dependent reduction in cell invasion provoked by TGF-1. Curcumin also markedly inhibited TGF-1-regulatedMMP-9 and activation of Smad2, ERK1/2 and p38 in a dose- and time-dependent manner. Additionally, PD98059,but not SB203580, showed a similar pattern of inhibition of MMP-9 expression. Conclusion: Curcumin inhibitedTGF-1-stimulated MMP-9 and the invasive phenotype in MDA-MB-231 cells, possibly associated with TGF-/Smad and TGF- /ERK signaling.

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