فایل ورد (word) مقاله Inhibitory Effects of ?-Cyclodextrin-Helenalin Complexes on H-TERT Gene Expression in the T47D Breast Cancer Cell Line - Results of Real Time Quantitative PCR

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 فایل ورد (word) مقاله Inhibitory Effects of ?-Cyclodextrin-Helenalin Complexes on H-TERT Gene Expression in the T47D Breast Cancer Cell Line - Results of Real Time Quantitative PCR دارای 8 صفحه می باشد و دارای تنظیمات در microsoft word می باشد و آماده پرینت یا چاپ است

فایل ورد فایل ورد (word) مقاله Inhibitory Effects of ?-Cyclodextrin-Helenalin Complexes on H-TERT Gene Expression in the T47D Breast Cancer Cell Line - Results of Real Time Quantitative PCR  کاملا فرمت بندی و تنظیم شده در استاندارد دانشگاه  و مراکز دولتی می باشد.

توجه : در صورت  مشاهده  بهم ريختگي احتمالي در متون زير ،دليل ان کپي کردن اين مطالب از داخل فایل ورد مي باشد و در فايل اصلي فایل ورد (word) مقاله Inhibitory Effects of ?-Cyclodextrin-Helenalin Complexes on H-TERT Gene Expression in the T47D Breast Cancer Cell Line - Results of Real Time Quantitative PCR،به هيچ وجه بهم ريختگي وجود ندارد


بخشی از متن فایل ورد (word) مقاله Inhibitory Effects of ?-Cyclodextrin-Helenalin Complexes on H-TERT Gene Expression in the T47D Breast Cancer Cell Line - Results of Real Time Quantitative PCR :


سال انتشار : 2013

تعداد صفحات :8

Background: Nowadays, the encapsulation of cytotoxic chemotherapeutic agents is attracting interest as amethod for drug delivery. We hypothesized that the efficiency of helenalin might be maximized by encapsulationin -cyclodextrin nanoparticles. Helenalin, with a hydrophobic structure obtained from flowers of Arnicachamissonis and Arnica Montana, has anti-cancer and anti-inflammatory activity but low water solubility andbioavailability. -Cyclodextrin (-CD) is a cyclic oligosaccharide comprising seven D-glucopyranoside units,linked through 1,4-glycosidic bonds. Materials and Methods: To test our hypothesis, we prepared -cyclodextrinhelenalincomplexes to determine their inhibitory effects on telomerase gene expression by real-time polymerasechain reaction (q-PCR) and cytotoxic effects by colorimetric cell viability (MTT) assay. Results: MTT assayshowed that not only -cyclodextrin has no cytotoxic effect on its own but also it demonstrated that -cyclodextrinhelenalincomplexes inhibited the growth of the T47D breast cancer cell line in a time and dose-dependent manner.Our q-PCR results showed that the expression of telomerase gene was effectively reduced as the concentrationof -cyclodextrin-helenalin complexes increased. Conclusions: -Cyclodextrin-helenalin complexes exertedcytotoxic effects on T47D cells through down-regulation of telomerase expression and by enhancing Helenalinuptake by cells. Therefore, -cyclodextrin could be superior carrier for this kind of hydrophobic agent.

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